Combined AFM and confocal fluorescence microscope for applications in bio-nanotechnology
نویسندگان
چکیده
منابع مشابه
Combined AFM and confocal fluorescence microscope for applications in bio-nanotechnology.
We present a custom-designed atomic force fluorescence microscope (AFFM), which can perform simultaneous optical and topographic measurements with single molecule sensitivity throughout the whole visible to near-infrared spectral region. Integration of atomic force microscopy (AFM) and confocal fluorescence microscopy combines the high-resolution topographical imaging of AFM with the reliable (...
متن کاملConfocal fluorescence microscopy with the tandem scanning light microscope.
Applications of the tandem scanning confocal microscope (TSM) to fluorescence microscopy and its ability to resolve fluorescent biological structures are described. The TSM, in conjunction with a cooled charge-coupled device (cooled CCD) and conventional epifluorescence light source and filter sets, provided high-resolution, confocal data, so that different fluorescent cellular components were ...
متن کاملUltrafast superresolution fluorescence imaging with spinning disk confocal microscope optics
Most current superresolution (SR) microscope techniques surpass the diffraction limit at the expense of temporal resolution, compromising their applications to live-cell imaging. Here we describe a new SR fluorescence microscope based on confocal microscope optics, which we name the spinning disk superresolution microscope (SDSRM). Theoretically, the SDSRM is equivalent to a structured illumina...
متن کاملTowards Optical Biopsies with an Integrated Fibered Confocal Fluorescence Microscope
This paper presents an integrated endoscope-compatible Fibered Confocal Fluorescence Microscope (FCFM) for medical imaging, the F400. In situ high resolution images can be obtained thanks to a set of flexible miniaturized optical probes of 0.5 to 1.5 mm diameter that can be inserted through the working channel of an endoscope. We briefly present in this paper the FCFM system, with a particular ...
متن کاملUltrastable combined atomic force and total internal fluorescence microscope
Combining atomic force microscope AFM with other microscopy techniques has expanded the range of potential applications for single molecule investigations dramatically. Particularly hybrid instruments with total internal reflection fluorescence TIRF excitation have opened new routes in life sciences. Here we present a novel design for such a hybrid microscope, which overcomes the limitations of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Microscopy
سال: 2005
ISSN: 0022-2720,1365-2818
DOI: 10.1111/j.0022-2720.2005.01428.x